ABSTRACT
BACKGROUND@#Islet transplantation is currently considered the most promising method for treating insulin-dependent diabetes. The two most-studied artificial islets are alginate-encapsulated b cells or b cell spheroids. As three-dimensional (3D) models, both artificial islets have better insulin secretory functions and transplantation efficiencies than cells in twodimensional (2D) monolayer culture. However, the effects of these two methods have not been compared yet. Therefore, in this study, cells from the mouse islet b cell line Min6 were constructed as scaffold-free spheroids or alginate-encapsulated dispersed cells. @*METHODS@#MIN6 cell spheroids were prepared by using Agarose-base microwell arrays. The insulin secretion level was determined by mouse insulin ELISA kit, and the gene and protein expression status of the MIN6 were performed by Quantitative polymerase chain reaction and immunoblot, respectively. @*RESULTS@#Both 3D cultures effectively promoted the proliferation and glucose-stimulated insulin release (GSIS) of MIN6 cells compared to 2D adherent cells. Furthermore, 1% alginate-encapsulated MIN6 cells demonstrated more significant effects than the spheroids. In general, three pancreatic genes were expressed at higher levels in response to the 3D culture than to the 2D culture, and pancreatic/duodenal homeobox-1 (PDX1) expression was higher in the cells encapsulated in 1% alginate than that in the spheroids. A western blot analysis showed that 1% alginate-encapsulated MIN6 cells activated the phosphoinositide 3-kinase (PI3K)/serine/threonine protein kinase (AKT)/forkhead transcription factor FKHR (FoxO1) pathway more than the spheroids, 0.5% alginate-, or 2% alginate-encapsulated cells did. The 3D MIN6 culture, therefore, showed improved effects compared to the 2D culture, and the 1% alginate-encapsulated MIN6 cells exhibited better effects than the spheroids. The upregulation of PDX1 expression through the activation of the PI3K/AKT/FoxO1 pathway may mediate the improved cell proliferation and GSIS in 1% alginate-encapsulated MIN6 cells. @*CONCLUSION@#This study may contribute to the construction of in vitro culture systems for pancreatic islets to meet clinical requirements.
ABSTRACT
This article systematically reviews the research results related to the machine learning based suicide ideation prediction on social networking platforms， so as to provide references for group and individual suicide prediction. This article will address the current states （issues of algorithm accuracy and efficiency， privacy leakage and stigma） and limitations of machine learning based suicide prediction on different platforms （light blogging， acquaintance social platforms， forums， picture and video sharing applications and clinical databases）.
ABSTRACT
<p><b>OBJECTIVE</b>This study aimed to investigate the current situation of parental rearing patterns and self-consciousness of adolescents with cleft lip and palate. The relationship between parental rearing patterns and self-consciousness of adolescents was also analyzed to provide evidence for psychological intervention.</p><p><b>METHODS</b>The study was carried out on 400 adolescents (11-25 years old) with cleft lip and palate. The subjects were assessed with self-consciousness scale for patients with cleft lip and palate and Egna Minnen av Barndoms Uppforstran family rearing questionnaires to evaluate the current status of self-consciousness and parental rearing patterns in adolescents with cleft lip and palate and the relevance of the two factors.</p><p><b>RESULTS</b>A total of 380 valid questionnaires were obtained. A significant relationship was found between parental rearing patterns and self-consciousness in adolescents with cleft lip and palate. A positive correlation was also observed between the scores of self-evaluation, self-confidence, others' evaluation and psychological endurance, interpersonal relationship, and parent-child relationship of adolescents with cleft lip and palate and the scores of their parents' emotional care. Confidence was negatively correlated with the scores of parents' refusal to deny, punishment, and severity.</p><p><b>CONCLUSIONS</b>Good parental rearing patterns play a critical role in establishing self-confidence, improving social adaptability, and maintaining interpersonal and parent-child relationship. Parents should give much care while avoiding refusal, denial, and punishment.</p>